不同临床类型紫癜性肾炎患儿白细胞介素10基因表达的研究

Expression of Interleukin-10 mRNA in Children with Different Clinical Types of Henoch-Schonlein Purpura Nephritis

目的：探讨白细胞介素１０（ＩＬ－１０）在不同临床类型紫癜性肾炎（ＨＳＰＮ）发病中的作用。方法：应用逆转录－多聚酶链反应（ＲＴ－ＰＣＲ）及双抗体夹心ＥＬＩＳＡ法检测了４０例ＨＳＰＮ患儿活动期和恢复期外周血单个核细胞（ＰＢＭＣ）在植物血凝素（ＰＨＡ）刺激下ＩＬ－１０ｍＲＮＡ和蛋白水平的变化。结果：①ＨＳＰＮ活动期ＰＢＭＣ中ＩＬ－１０ｍＲＮＡ和蛋白水平均显著高于正常对照组［（０．４８±０．１２）比（０．３９±０．１０）和（１０２３．９０±１５８．７８）ｐｇ／ｍｌ比（８９５．５１±１４１．０６）ｐｇ／ｍｌ，Ｐ分别＜０．０１及０．０５］，恢复期与正常对照组相比差异无显著性［（０．４２±０．１３）比（０．３９±０．１０）和（９４１．２５±１６５．３６）ｐｇ／ｍｌ比（８９５．５１±１４１．０６）ｐｇ／ｍｌ，Ｐ均＞０．０５］。②临床上表现为蛋白尿型、肾病综合征型及急性肾炎综合征型者，活动期ＰＢＭＣ中ＩＬ－１０ｍＲＮＡ和蛋白水平均显著高于正常对照组，而表现为单纯血尿型者与正常对照组相比差异无显著性。结论：ＨＳＰＮ患儿活动期ＩＬ－１０表达异常且与临床类型有一定的关系。ＩＬ－１０在其发病中具有一定的保护作用。

Objective:To investigate the role of interleukin-10 (IL-10) in the pathogenesis of different clinical types Henoch-Schonlein purpu ra nephritis (HSPN). Methods:The IL-10 protein was determined by ELIS A and the expression of IL-10 mRNA was measured by reverse transcription-polym eras e chain reaction (RT-PCR) in phytohemagglutinin (PHA)-stimulating peripheral b lood mononuclear cells (PBMCS) in 40 children with HSPN during their acute phase and remission stage. Results:①The levels of IL-10 mRNA and protein w ere significantly increased in patients in acute phase compared with controls ［ (0 .48±0.12) vs (0.39±0.10) and (1023.90±158.78) pg/ml vs (895.51±141.06) p g/ml ; %P%<0.01 and 0.05 respectively］, while in remission stage the levels of IL -10 decr eased to normal levels ［(0.42±0.13) vs (0.39±0.10) and (941.25±165.36) pg/ml vs (895.51±141.06) pg/ml; %P%>0.05 for both］. ②The IL-10 mRNA and pro tein of HSPN p atients in acute phase with proteinuria, nephrotic syndrome or acute glomerulone phritis syndrome was signficantly higher than that in controls. There was no sig nificant difference between the acute phase of HSPN patients with isolated hemat uria and the controls. Conclusion:The results suggested that the expre ssion of IL-10 in PBMC was correlated to clinical type of HSPN. IL-10 has prot ective action in the pathogenesis of HSPN.