胃粘膜活检标本端粒酶活性的检测及其临床意义

Telomerase Activity in Gastric Mucosa Obtained from Endoscopy and Clinical Significance

目的：检测胃粘膜病变组织的端粒酶活性，探讨端粒酶活化在胃癌发生发展中的作用，并阐明它的临床意义。方法：采用非放射性的ＰＣＲ－ＥＬＩＳＡ法检测胃粘膜活检标本的端粒酶活性。结果：９３例胃粘膜活检标本中，慢性浅表性胃炎、慢性萎缩性胃炎、肠上皮化生、异型增生及胃癌的端粒酶阳性率分别为０％（０／１０）、２８．１３％（９／３２）、１６．６７％（２／１２）、３０．００％（３／１０）、８６．２１％（２５／２９）。胃癌组织端粒酶阳性率与性别、肿瘤部位、大体类型及组织学分类无明显相关。结论：端粒酶活性的检测对胃癌的诊断可能具有重要意义，有望成为一种理想的胃癌标志物。

Objective: To determine the telomerase activity of ga stric cancer and evaluate its relation with the genesis of gastric carcinoma.Methods: Telomerase activity in gastric mucosa obtained from endoscopy wa s detected by method of non-radioisotopic PCR-ELISA.Results: The tel omerase activity of 93 specimens of gastric mucosa was various with different hi stopathology. The positive rate in chronic superficial gastritis(CSG) was 0%(0/1 0), in chronic atrophic gastritis (CAG) was 28.13%(9/32), in intestinal metaplas ia was 16.67%(2/12), in intestinal dysplasia was 30.00%(3/10), in gastric cancer was 86.12%(25/29). Telomerase activity in gastric cancers had no relation with gender, tumor location and macroscopic and microscopic morphology. Conclus ion: Telomerase activity may serve as a molecular biological marker for diag nosis of gastric carcinoma.