异种肝细胞移植后受体血清白细胞介素2及肿瘤坏死因子α水平的研究

A Preliminary Study on Serum Interleukin-2(IL-2) and Tumor Necros is Factor Alpha (TNFα) in Rats after Xenogeneic Hepatocyte Transplantation

目的：通过测定异种肝细胞脾内移植后受体血清白细胞介素２（ＩＬ－２）和肿瘤坏死因子α（ＴＮＦα）的浓度，初步探讨其排斥反应机理。方法：向用Ｄ－氨基半乳糖（Ｄ－ＧＩ）诱导的肝衰大鼠脾内移植豚鼠肝细胞。脾内注入生理盐水为对照。观察２周生存率，在术后不同时间作受体脾病理组织学检查及酶联免疫双抗体夹心法（Ｓａｎｄｗｉｃｈ－ＥＬＩＳＡ）测定大鼠血清ＩＬ－２和ＴＮＦα的浓度。结果：２周生存率比较，移植组（８０．０％）显著高于对照组（２１．４％，Ｐ＜０．０５）。移植的肝细胞在移植早期（１～２天）消失，移植后第３天炎症细胞浸润显著增多。移植组与对照组大鼠血清ＩＬ－２、ＴＮＦα浓度均无显著差别（Ｐ＞０．０５）；与正常大鼠比较，差异亦无显著性（Ｐ＞０．０５）。结论：在异种肝细胞脾内移植中，细胞免疫的发生较迟和（或）较弱，移植受体血清ＩＬ－２及ＴＮＦα的浓度无显著变化。

Objective:To study the rejection mechanisms of xenogeneic hepatocyte transplantation (XHT), the levels of serum interleukin -2(IL- 2) and tumor necrosis factor alpha (TNF-α) were detected after XHT. Meth ods:The hepatic failure rats induced by D-galactosamine (D-GI) were divide d into two groups, the transplanted group and control group. The former was tran splanted with hepatocytes of Guinea-pig into their spleens. The 2-week surviva l rate in both groups after XHT was observed. The histological examinations of s pleens and the measure of IL-2 and TNFα in rats serum by Sandwich-ELISA meth od were carried out in different time. Results:The 2-week survival ra te in transplanted group (80%) was higher than that in control group (21.4%, P＜0.05). Grafted hepatocytes disappeared shortly after transplantation and a lot of inflamantary cells infiltrated at the 3rd day after transplantation. Ther e was no significant difference in serum IL-2 and TNF α of the rats among the norm al, transplanted group and control group (P＞0.05). Conclusion:Cel lular immune response may occur later and /or be feeble and the serum IL-2 and TNF α have no significant change in XHT.