摘要
目的:观察大鼠中缝背核(DR)内一氧化氮合酶(NOS)阳性神经元向中央杏仁核(CeA)的投射,为进一步阐明一氧化氮(NO)参与情绪、疼痛、内脏心血管活动的中枢调节机制提供形态学资料。方法:用黄递酶(NADPH-d)组织化学染色结合辣根过氧化酶(HRP)逆行追踪技术,观察大鼠DR内向CeA投射的NOS阳性神经元的分布与形态。结果:DR内有一定数目的NOS/HRP双标阳性神经元,主要分布在外侧部与背正中部。结论:DR内有部分NOS阳性神经元向CeA投射,提示NO在DR至CeA上行感觉传导通路中起重要的神经递质作用。
Objective:To provide the morphological data for furth er understanding the role of nitric oxide(NO) in central modulation of pain, moo d and visceral and cardiovascular activity, we studied nitric oxide synthase(NOS ) positive neurons of the dorsal raphe nucleus(DR) projecting to the central amy gdalagy nucleus(CeA) in rat. Methods: Localization of DR NOS positive neurons projecting to CeA was evaluated by nicotinamide adenine dinucleotide pho sphate naloxone diaphorase histochemical technique combining with horseradish pe roxidase(HRP) as a retrograde tracer. Results: A few NOS/HRP double-l abeled neurons were found in the DR, it majority was distributed at the lateral and the dorsal median part. Conclusion: The DR has some NOS positive neurons projecting to the CeA. It suggests that NO may act as an important neuro transmitter in the ascending sensory pathways of the DR.
出处
《南京医科大学学报(自然科学版)》
CAS
CSCD
北大核心
2002年第2期132-134,I001,共4页
Journal of Nanjing Medical University(Natural Sciences)
基金
南京医科大学科技发展基金资助项目(NY99006)
关键词
中缝背核
中央杏仁核
一氧化氮合酶
投射
大鼠
阳性神经元
the dorsal raphe nucleus
the central amygdalagy nucle us
nitric oxide synthase
projection
rat