大鼠中缝背核内一氧化氮合酶阳性神经元向中央杏仁核的投射

Nitric Oxide Synthase Positive Neurons of the Dorsal Raphe Nucleus Projecting to the Central Amygdalagy Nucleus in Rat

目的：观察大鼠中缝背核（ＤＲ）内一氧化氮合酶（ＮＯＳ）阳性神经元向中央杏仁核（ＣｅＡ）的投射，为进一步阐明一氧化氮（ＮＯ）参与情绪、疼痛、内脏心血管活动的中枢调节机制提供形态学资料。方法：用黄递酶（ＮＡＤＰＨ－ｄ）组织化学染色结合辣根过氧化酶（ＨＲＰ）逆行追踪技术，观察大鼠ＤＲ内向ＣｅＡ投射的ＮＯＳ阳性神经元的分布与形态。结果：ＤＲ内有一定数目的ＮＯＳ／ＨＲＰ双标阳性神经元，主要分布在外侧部与背正中部。结论：ＤＲ内有部分ＮＯＳ阳性神经元向ＣｅＡ投射，提示ＮＯ在ＤＲ至ＣｅＡ上行感觉传导通路中起重要的神经递质作用。

Objective:To provide the morphological data for furth er understanding the role of nitric oxide(NO) in central modulation of pain, moo d and visceral and cardiovascular activity, we studied nitric oxide synthase(NOS ) positive neurons of the dorsal raphe nucleus(DR) projecting to the central amy gdalagy nucleus(CeA) in rat. Methods: Localization of DR NOS positive neurons projecting to CeA was evaluated by nicotinamide adenine dinucleotide pho sphate naloxone diaphorase histochemical technique combining with horseradish pe roxidase(HRP) as a retrograde tracer. Results: A few NOS/HRP double-l abeled neurons were found in the DR, it majority was distributed at the lateral and the dorsal median part. Conclusion: The DR has some NOS positive neurons projecting to the CeA. It suggests that NO may act as an important neuro transmitter in the ascending sensory pathways of the DR.