肝细胞癌乙型肝炎病毒感染与人胎盘型谷胱甘肽S-转移酶的表达

Expression of GST-π and HBV Infection in Hepatocellular Carcinoma

背景与目的:许多肿瘤癌变过程中人胎盘型谷胱甘肽S-转移酶(glutathioneS-transferases,GST-π)的表达会异常升高,其变化早于细胞的形态改变。探讨肝细胞癌乙型肝炎病毒(hepatitisBvirus,HBV)感染与GST-π表达的关系。方法:对肝细胞癌和相关慢性肝病及正常肝组织共86例用免疫组化染色方法检测乙型肝炎表面抗原(hepatitisBsurfaceantigen,HBsAg)、乙型肝炎核心抗原(hepatitisBcoreantigen,HBcAg)和GST-π,用原位分子杂交方法检测乙型肝炎病毒DNA(hepatitisBvirusDNA,HBVDNA)。结果:HBsAg,HBcAg和HBVDNA的阳性率在慢性肝炎分别为61.9%(13/21);42.9%(9/21)和75.0%(12/16);在肝硬化分别为64.0%(16/25),36.0%(9/25)和83.3%(15/18);在癌旁肝硬化分别为72.7%(16/22),61.1%(11/18)和85.7%(12/14);在肝细胞癌分别为45.2%(14/31),50.0%(14/28)和64.3%(9/14)。其中以癌旁肝硬化组阳性率最高。慢性肝炎、肝硬化和癌旁肝硬化HBVDNA阳性信号较肝细胞癌多而强。GST-π在慢性肝炎(25.0%,4/16)、肝硬化(17.6%,3/17)、癌旁肝硬化(53.3%,8/15)和肝细胞癌(60.0%,9/15)均有表达,但癌旁肝硬化组和肝细胞癌组阳性率明显增高,癌旁肝硬化组与不伴肝癌的肝硬化组差异有显著性(P<0.05),与肝细胞癌组差异无显著性(P>0.05)。结论:大多?

Background & Objective: The expression of glutathione S-transferases(GST-π) might abnormally increase in many carcinogenesis, and the alteration of GST π preceded than that alteration of cell morphology. This study was designed to investigate the expression of glutathione S-transferases (GST-π) and its relationship with hepatitis B virus (HBV) infection in hepatocellular carcinoma (HCC). Methods: Hepatitis B surface antigen (HBsAg), Hepatitis B core antigen (HBcAg), and GST-π were dected by immunohistochemical staining and HBV DNA was detected by in situ hybridization (ISH) in total 86 samples of chronic hepatitis, liver cirrhosis, paratumor cirrhosis, HCC, and normal liver tissue. Results: The positive expression rates of HBsAg, HBcAg, and HBV DNA were 61.9%(13/21), 42.9%(9/21), and 75.0%(12/16) in chronic hepatitis;64.0%(16/25),36.0%(9/25), and 83.3%(15/18) in liver cirrhosis; 72.7%(16/22),61.1%(11/18), and 85.7%(12/14) in the paratumor cirrhosis,as well as 45.0%(14/31), 50.0%(14/28) and 64.3%(9/14) in HCC. HBV DNA positive granules in chronic hepatitis, liver cirrhosis, and the paratumor cirrhosis were more and stronger than that in HCC, respectively. The positive expression rates of GST-π were 25.0%(4/16),17.6%(3/17),53.3%(8/15), and 60.0%(9/15) in chronic hepatitis, liver cirrhosis, the Paratumor cirrhosis, and HCC, respectively. The positive rate of GST π in the paratumor cirrhosis was significantly higher than that in the liver cirrhosis without tumor (P< 0.05), but the same as in HCC (P >0.05). Conclusions: Most of the HCC cases were closely associated with chronic hepatitis and liver cirrhosis of HBV infection. The HBV infecation may increase expression of GST π. The paratumor cirrhosis may be a sequential lesion of precancerous cirrhosis around HCC.