去卵巢大鼠骨质疏松经治疗后骨组织计量学的研究

Histomorphometric analysis in ovariectomized rat with osteoporosis

目的 :建立大鼠骨质疏松模型 ;探讨去卵巢后雌性大鼠骨丢失的组织学机制及尼尔雌醇和左炔诺孕酮的治疗作用。方法 :通过图像分析仪对骨组织进行形态计量学分析。结果 :与假手术组比较 ,去卵巢大鼠组相对骨小梁体积、骨小梁平均厚度及骨小梁周长均显著减少 (P <0 .0 1) ,而成骨细胞指数、破骨细胞指数、骨小梁吸收表面和成骨细胞覆盖骨小梁表面百分比均明显增加 (P <0 .0 1)。尼尔雌醇和左炔诺孕酮治疗组上述各指标接近于假手术组 (P >0 .0 5 )。结论 :本实验去卵巢大鼠骨质疏松模型成立 ;去势后雌性大鼠骨丢失乃骨转换加快 (高转换型 )和重建负平衡 (骨吸收超过骨形成 )所致 ;用尼尔雌醇和左炔诺孕酮治疗能降低这种骨转换的负平衡 ,达到治疗骨质疏松的目的。

Objective To establish the pattern of bone loss of Sprague-Dawley rats after ovariectomy, to investigate and assess the pattern of bone remodeling in ovariectomized rats and the therapeutic action of nilestriol and levenorgestrel. Methods Histomorphometric analysis for bone tissues was performed with the instrument of computerized image analysis. Results The trabecular bone volume, mean trabecular thickness, and trabecular bone perimeter were all significantly decreased (P<0.01) in ovariectomized rats in the 14th week postsurgery compared to the sham operated group. But the osteoblastic index, osteoclastic index, resorptive surface of trabecular , and trabecular surface covered by osteoblast were significantly increased (P<0.01). There was no significant difference between the treatment group and the sham operated group. Conclusions: The pattern of bone loss of rats after ovariectomy has been established successfully. The study provides evidence that the cause of bone loss in the ovariectomized rats is attributable to the increase of bone turnover and negative imbalance in bone remodeling process. The treatment of nilestriol and levenorgestrel may decrease the bone turnover and correct the imbalance to cure the osteoporosis.