黑曲糖化酶糖化生玉米粉酒精发酵初探

Preliminary Study on Alcoholic Fermentatoin of Uncooked Corn Meal by Using Glucoamylase

本文研究了利用黑曲糖化酶糖化生玉米粉进行酒精发酵的新工艺。该技术不需要加热糊化和添加α-淀粉酶液化,仅要求糖化酶用量为500单位/每克玉米粉即可达到与成熟的玉米粉80℃低温蒸煮和高压蒸煮酒精生产技术相同的发酵水平。但因糖化酶中含有转苷酶,发现如过多添加糖化酶会引起酒精产率下降。进一步的经济效益估算表明,生玉米粉酒精发酵技术虽然节省了大量能源,简化了操作工序,但要在工业上推广应用,关键还在于要降低生产成本。

This paper studies the new technology of alcoholic fermentation of uncooked corn meal by using glucoamylase derived from Aspergillus niger is studied. With this technology,it is unnecessary to gelatinate corn starch by heating and to liquefy corn starch with α-amylase. And only adding of 500 units of the glucoamylase per gram corn meal, same result will be shown like that of the present 80℃ corn meal low-temperature cooking and high-pressure cooking. It is found that the yield of ethanol from corn meal will be reduced if more glucoamylase, in which transglucosidase exists, is used,The estimated economic result showes that the key to expend the technology in large scale lies in reducing production cost,although the new technology has the advangtages of energy-saving, easy operation.