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快速构建腺病毒载体的新方法 被引量：1

The Simple Methods for Construction of Adenovirus Vectors
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摘要 目前腺病毒载体已成为重要的基因治疗载体 ,随着分子生物学技术的迅速发展 ,构建重组腺病毒的方法有了很大改进 ,本文主要介绍 3种快速构建腺病毒载体的新方法 :一是细菌内同源重组法构建重组腺病毒 ;二是细胞外连接法构建重组腺病毒 ;三是在黏粒中构建重组腺病毒。 3种构建腺病毒载体的新方法相比于传统方法 ,具有简便、快速 ,实验周期短的优点。
作者 赵峰
机构地区 四川大学华西医院肿瘤中心胸外科
出处 《中国肿瘤生物治疗杂志》 CAS CSCD 2001年第4期309-311,共3页 Chinese Journal of Cancer Biotherapy
基金 国家自然科学基金 ( 30 0 0 0 2 0 6 )项目资助
关键词 腺病毒 腺病毒载体 构建 基因治疗 肿瘤
分类号 R730.5 [医药卫生—肿瘤]
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参考文献12

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同被引文献14

  • 1冯霞,余双庆,陈国敏,左建民,周玲,曾毅.含密码子优化型HIV-1 gp120基因重组腺病毒的构建及其免疫效果研究[J].中华实验和临床病毒学杂志,2004,18(2):113-117. 被引量：3
  • 2汤福祥,郑权,黄宗海,张庆国,车小燕.应用改进的AdEasy系统制备重组腺病毒[J].第一军医大学学报,2003,23(5):501-503. 被引量：14
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中国肿瘤生物治疗杂志
2001年 第4期

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