人骨肉瘤细胞系OS-732CAM移植瘤中VEGF、bFGF的表达及意义

Expression of VEGF and bFGF in xenografts of human osteosarcoma OS-732 on chick embryo chorioallantoic membrane and its significance

目的 :研究人骨肉瘤细胞系OS 732血管生成相关作用。方法 :应用鸡胚绒毛尿囊膜模型 ,通过解剖显微镜及透射电镜观察该细胞系促血管生成特点 ,并以免疫组化方法检测人骨肉瘤细胞系OS 732鸡胚绒毛尿囊膜移植瘤中血管内皮生长因子 (vascularendothelialgrowthfactor,VEGF)、碱性成纤维细胞生长因子 (Basicfibroblastgrowthfactor ,bFGF)的表达。结果 :该细胞系具有较强的诱导血管生成能力 ,解剖显微镜下可见血管辐辏现象 ,透射电镜下可见新生血管壁由单层内皮细胞构成 ,内皮细胞裂隙增宽 ,基底膜不完整 ,缺乏平滑肌成分 ,移植瘤组织中VEGF和bFGF均呈阳性表达 ,其中VEGF呈高表达。结论 :肿瘤诱导的新生血管在病理、生理及形态功能方面都具有特征性 ,其诱导血管生成过程中可能有多种血管生长因子的共同参与 ,针对血管生长因子为靶点进行抗血管生成治疗对改善骨肉瘤预后可能具有重要意义。

Purpose:To study the characterics of angiogenesis induced by osteosarcoma OS-732 cell line. Methods:With a tumor model of the chick embryo chorioallantoic membrane(CAM),the angiogenesis induced by OS-732 cell line was observed by a stereo-microscope and transmission electron microscope, and the expression of angiogenic factors vascular endothelial growth factor(VEGF) and basic fibroblast growth factor(bFGF) in xenografts of OS-732 cell line on CAM was detected by immunohistochemical technique. Results:This cell line was strongly angiogenic, which probably is associated with production of many factors.The new capillaries converging upon the tumor can be observed by a stereo-microscope. Furthermore,under transmission electron microscope, it also can be noted that the new blood vessel consisted of a monolayer of vascular endothelial cells with enlarged fissures,and the basal membrane was not intact. The expression of VEGF and bFGF was positive in xenografts on CAM, and VEGF was expressed at high levels constantly. Conclusions:The new blood vessels induced by tumors have pathologic and physiopathologic characteristics and many angiogenic factors are alm involved in the angiogenic process of OS-732. Furthermore, the application of angiogenic factors as a target in antiangiogenic therapy of osteosarcoma may be useful to improve the prognosis of patients with this disease.