采用固定化酵母以带渣的薯干糖化醪进行酒精发酵的研究

Study on Alcoholic Fermentation from the Mash of Dried Sweet Potato with Its Dregs by Immobilized Yeast Cells

研制了海藻酸钙膜片状固定化酵母填充床生物反应器,以带渣的低温蒸煮薯干糖化醪为基质,进行酒精发酵试验,获得了如下结果:淀粉利用率达90％;生物反应器的乙醇生产能力可达32mg乙醇/g凝胶·小时,以床层体积计为9.8g乙醇/l反应器·小时,为传统式酒精发酵罐乙醇生产能力的12倍;操作性能良好,反复活化运转三个月以上,发酵活力未见明显下降。

A packed-bed bioreactor is developed. This reactor has a packed layers of membrane pieces from with immobilized yeast cells which are entraped in calcium alginate. The fermentation substrate is a mash of dried sweet potato with its dregs, which is prepared from low temperature cooking process. The following results are obtained, the utilization rate of starch is 90%; the alcohol productivity of bioreactor is 32mg EtOH/g-gel·h; and the volume productivity according to the volume of packed-bed is 9.8g EtOH/l·h, which is equal to 12 times that is the productivity of conventional batch fermentation) the operation stability of bioreactor is well, its fermentation activity does not decrease obviously after operation more than three months.