星形细胞肿瘤中端粒酶与PTEN的表达及相关性

Relationship between telomerase and PTEN expression in astrocytoma

目的 :研究星形细胞肿瘤中的端粒酶活性和PTEN表达 ,探讨其在星形细胞肿瘤发生发展过程中的相关性。方法 :收集手术切除经病理证实的星形细胞肿瘤 110例 ,8例正常脑组织作对照。用端粒酶原位杂交检测盒检测组织标本端粒酶活性。用LSAB法检测PTEN表达。结果 :星形细胞肿瘤端粒酶活性和PTEN的阳性检出率分别为 5 0 % (5 5 / 110 )和 39 1%(4 3/ 110 )。且随着肿瘤恶性程度的增加 ,端粒酶活性程度升高 (P <0 0 1)。端粒酶活性与PTEN表达有相关性 (P <0 0 1)。结论 :端粒酶活性与星形细胞肿瘤的分化程度有关 ,提示端粒酶活性可能是星形细胞肿瘤恶性程度的重要标记物 。

Purpose To study the telomerase activity and expression of PTEN protein in astrocytoma and to investigate their relations during histogenesis and development of astrocytoma. Methods Totally 110 cases of astrocytoma tissues and 8 normal brain tissues were studied. The telomerase activity was detected using telomerase ISH detection Kit. PTEN protein was detected by immunohistochemical LSAB method. Results The positive staining rates of telomerase and PTEN protein in astrocytoma were 50%(55/110) and 39 1% (43/110) respectively. Expression of telomerase was positively correlated with the malignant degree of astrocytomas ( P <0 01). Telomerase activity was associated with PTEN expression in astrocytomas ( P <0 01). Conclusions Telomerase activity correlates with the degree of the neoplastic differentiation, and may be an important marker for the malignancy of astrocytoma. PTEN may affect telomerase activity.