不同原因诱导大鼠腹主动脉移植硬化的组织病理比较

Histopathology of rat aortic transplant arteriosclerosis induced by different risk factors

目的 :研究不同原因诱导的大鼠腹主动脉移植硬化的组织病理学改变。方法 :140只大鼠随机分组 :A组为非移植组 ,B组为同品系移植缺血小于 30min组 ;C组为同品系移植缺血 4h组 ;D组为异品系移植缺血小于 30min组。术后 7、15、30、6 0天切取植入的腹主动脉进行光镜、电镜检查。结果 :移植 7～ 15天时C组动脉内膜有多量多形核粒细胞和单个核细胞粘附、浸润 ,电镜检查多形核粒细胞为中性粒细胞。而D组移植动脉各层均有大量的淋巴细胞浸润。移植 30～ 6 0天时C组、D组移植动脉内膜均有显著增厚 ,增生的内膜均由单核 /巨噬细胞和平滑肌细胞构成 ,但前者无中膜层平滑肌坏死及弹力膜断裂现象。结论 :中性粒细胞在缺血 /再灌注损伤诱导的同品系大鼠腹主动脉移植硬化发挥了重要作用 ,其机制值得进一步研究。

Purpose To investigate the histopathology of rat aortic transplant arteriosclerosis induced by different cause. Methods One hundred and forty inbred SD and Wistar rats were randomly divided into the nontransplanted control group (A), the ischemia control group of isografts (B), the ischemia experimental group of isografts (C), the allografts group (D). Grafts were harvested at 7, 15, 30, 60 days after transplantation and studied by light and electron microscopy. Results At the early observation time after transplantation, the cell composition found in the intima of the syngenic vessels with 4 hours of ischemia time consisted of neutrophils and monocytes. Whereas in allogeneic grafts it contained lymphocytes and monocytes. Dominant intimal thickening was found in allogeneic grafts and syngenic grafts with 4 hours of ischema time during the late observation time. The thickening neointimae consisted mainly of monocyte/macrophage and smooth muscle cells (SMC). Conclusions The damage due to prolonged cold ischemia time is sufficient to cause pronounced graft arteriosclerosis. The pathophysiological mechanism leading to ischemia induced arteriosclerosis is different from the one seen in the allogeneic situation.