β-七叶皂苷钠对大鼠脑出血后脑水肿及脑内精氨酸加压素含量的影响

Effects of β-sodium aescinate on hemorrhage brain edema and AVP contents in rats

目的 :观察 β-七叶皂苷钠治疗前后大鼠脑出血各脑区与垂体精氨酸加压素 ( AVP)含量的变化。方法 :采用立体定向于大鼠尾壳核注射胶原酶建立脑出血动物模型 ,以干湿重法测定脑组织含水量 ,放射免疫法测定 AVP含量。结果 :与正常组及对照组相比 ,脑出血组大鼠在各脑区脑组织含水量显著升高的同时 ,AVP含量也显著升高 ,而且下丘脑与垂体的 AVP含量也显著升高 ;β-七叶皂苷钠治疗后大鼠脑组织含水量显著降低 ,顶叶皮质、下丘脑及垂体 AVP含量也显著降低。各项结果均有显著性差异 ( P<0 .0 5 ,P<0 .0 1)。结论 :内源性 AVP可能参与脑出血后脑水肿的病理过程 ,β-七叶皂苷钠抗脑水肿的药理作用机制中可能有 AVP参与。

Objective: To observe the content of AVP changes in brain regions and pituitary of intracerebral hemorrhagic rats before and after treatment by β sodium aescinate. Methods: Animal model was established by injecting collagenase into left caudoputamen of adult rats. Radioimmunoassay(RIA) was performed to measure the content changes of AVP, dye wet Weight methods was used to measure the changes of water contents in brain. Results: Compared with control group, the water content was increased significantly ( P <0.01), AVP also increased significantly( P <0.05, P <0.01), furthermore, the contents of AVP in hypothalamus and pituitary increased too( P <0.05, P <0.01). After treated by β sodium aescinate, the water contents in brain regions,the contents of AVP in parietal cortexs,hypothalamus and pituitary decreased significantly. Conclusion: AVP may be related to process of brain edema after intracerebral hemorrhage, AVP has pharmaceutical effects against brain edema.