CTLA- 4 Ig对大鼠类风湿性关节炎的负性调节作用

Role of CTLA-4 Ig in rat arthritis models

目的 :探讨 CTLA-4 Ig在类风湿性关节炎发病机制中的负性调节作用。 方法 :经牛 型胶原 ( B C)诱导 SD大鼠建立大鼠关节炎模型 ;以抗原刺激培养的淋巴结细胞 ( LNC)后 ,用改良的 MTT法检测 L NC转化反应 ,ELISA法测定 L NC培养上清中的 IL -2和 IFN-γ分泌量及 B C特异性抗体 ;观察 CTLA-4 Ig对 L NC转化及细胞因子分泌的影响。 结果 :CTL A-4 Ig可阻止 SD大鼠发生胶原诱发性关节炎 ,可抑制 LNC二次免疫反应中的淋巴细胞转化、IL-2和 IFN-γ分泌、抗体产生。 结论 :B7:CD2 8/CTL A-4共刺激信号在诱发 T细胞介导的自身免疫病中起作用 ,CTL A-4

Objective: To study the role of CTLA 4 Ig in the pathogenesis of rheumatoid arthritis(RA). Methods: Firstly rat RA model was set up by injecting bovine collagen Ⅱ(BⅡC); then IL 2,IFN γ and anti BⅡC antibody were detected in the LNC culture with antigen stimulation by ELISA, and LNC proliferation was analyzed by MTT. Results: CTLA 4 Ig prevented the development of arthritis, proliferation of lymphocytes and production of IL 2, IFN γ and anti BⅡC antibody. Conclusion: B7:CD28/CTLA 4 costimulation plays a role in the autoimmune diseases and CTLA 4 Ig has potential therapeutic effect.