梅毒螺旋体特异抗原 P15、P47的克隆表达和临床应用

Cloning and expression of Treponema pallidum specific antigens P15 and P47 for clinical application

目的 :克隆表达梅毒螺旋体特异抗原 P15、P47,用于临床检测梅毒感染。方法 :化学合成 p15、p47基因并插入 GST融合蛋白表达载体 ,以亲和层析进行纯化。将重组 P15、P47抗原包板制备酶联免疫检测试剂盒 ,对国家标准品、正常人群、梅毒患者等的血清进行检测。结果 :化学合成的 p15、p47基因 ,测序结果与 Gen Bank数据符合。将重组 P15、P47抗原包板制备酶联免疫检测试剂盒 ,对国家标准品的检测全部符合 ,对正常人群、梅毒患者和其他一些疾病患者的检测显示很高的灵敏度和特异性。 结论 :用重组梅毒 P15和

Objective: To clone and express Treponema pallidum (TP) specific antigens P15 and P47 and use them in clinical examination. Methods: P15 and p47 genes synthesized were inserted into a GST fusion expression vector. The recombinant antigens were purified by affinity chromatography and then coated on microplates to establish an ELISA kit. Using this kit, national TP standards, sera of normal persons and patients with TP and other diseases were tested. Results: The synthesized p15 and p47 genes sequenced were identical with those of GenBank. National TP standards were tested by ELISA kit which coated with these recombinant antigens, and the results accorded with the standards. High sensitivity and specificity was showed when 2 recombinant antigens were used in ELISA to detect the sera of normal persons and patients with TP and other diseases. Conclusion: The recombinant TP specific antigen P15 and P47 are suitable for establishment of ELISA kit in clinical examination.