染料木素和槲皮素对成纤维细胞增殖和胶原合成的抑制作用

Inhibitive effects of genistein and quercetin on NIH3T3 fibroblast proliferation and collagen synthesis

目的 :研究染料木素 ( genistein,GE)和槲皮素 ( quercetin,QU)对成纤维细胞增殖和胶原合成的影响。方法 :用结晶紫染色法测定光密度值检测 NIH3T3成纤维细胞增殖 ,用 3 H-脯氨酸掺入法测定 3 H-脯氨酸掺入 NIH3T3细胞的 Bq值表示胶原合成作用。 结果 :GE和 QU都能浓度依赖性抑制血清、血小板源生长因子 ( PDGF )或转化生长因子β1( TGF-β1)刺激的NIH3T3细胞增殖和胶原合成。 结论 :GE和 QU具有体外抑制成纤维细胞增殖和胶原合成的作用。

Objective:To study the effects of genistein (GE) and quercetin (QU) on proliferation and collagen synthesis of mouse fibroblast NIH3T3 cells.Methods:Cell proliferation was measured by crystal violet staining assay.Collagen synthesis was determined by 3H proline incorporation assay.Results:GE (25 70 μmol·L -1 ) and QU (6.25 50 μmol·L -1 ) concentration dependently reduced the serum driven increase of cell proliferation and collagen synthesis.GE and QU also attenuated platelet derived growth factor (PDGF) driven proliferative activity and transforming growth factor β1 (TGF β1) driven collagen synthesis.Conclusion:GE and QU can inhibit mouse fibroblast NIH3T3 cell proliferation and collagen synthesis in vitro .