奥美拉唑对大鼠胃粘膜损伤保护作用及其机制的初步探讨

The Protective Effect of Omeprazole on the Gastric Mucosal Lesion in Rasts and it's Mechanisms

采用激光多普勒血流计 ( L DF)测定胃粘膜血流量 ( GMBF) ,镉粒还原和比色法测定胃粘膜和血浆 NO- 2 /NO- 3 含量 ,观察胃粘膜损伤指数( U I)、溃疡坏死组织和中性粒细胞浸润严重程度的变化。结果显示 ,与模型损伤组比奥美拉唑组大鼠 UI明显降低 ( P<0 .0 1) ,溃疡坏死组织和中性粒细胞浸润程度明显减轻 ( P<0 .0 1)。预先用 L-硝基 -精氨酸甲酯 ( L -NAME)处理后 ,奥美拉唑保护胃粘膜损伤作用明显减弱 ;L -NAME抑制作用可被 L -精氨酸拮抗。静脉注射奥美拉唑 ,可增加 GMBF、胃粘膜和血浆 NO- 2 /NO- 3,L -NAME可逆转这种作用。

Gastric mucosal blood folw(GMBF)was assessed with laser Doppler flowmetry (LDF),gastric mucosal and serum NO 2/NO 3 were measured by cadmium granulation reduction and colorimetric method,and the changes of ulcer index and the severity of tissues necrosis and neutrophils infiltration were observed. The results showed that ulcer index of the omeprazole group was much lower than control group(P<0 01),and the degree of tissues necrosis and meutrophils infiltration were much milder than that of the control group(P<0 01).The protective effect of omeprazole was significantly decreased by prior admindistration of Nw nitro L arginine methyl ester(L NAME).The inhibitory effect of L NAME was antagonized by prior administration of L arginine. Omeprazole administered into the vein obviously increased GMBF,gastric mucosal and serum NO 2/NO 3,this effect was prevented by pretreatment with L NAME.The results suggest that omeprazole plays an important protective role on the gastric mucosal lesion in rats via nitric oxide.