膝关节制动对外侧副韧带胶原转化影响的观察

Effect of immobilization on collagen transformation of lateral collateral ligament in knee

目的 探讨制动对鼠膝外侧副韧带胶原转化的影响。方法 将3H 脯氨酸注射入 5只大鼠体内 6周 ,然后将膝关节制动 8周 ,取膝外侧副韧带作液闪测定及氨基酸分析。结果 制动侧外侧副韧带的液闪测定值较对侧明显降低 ,与对照侧比较 ,差异有显著性 (P <0 .0 5 ) ,外侧副韧带得到新补充的胶原为 3 2 .3 5 % ,丢失的旧胶原为 4 2 .4 1% ,胶原净丧失 10 .0 5 % ,羟脯氨酸含量较对照侧明显减少。结论 制动后鼠膝外侧副韧带胶原的降解大于合成。

Objective The paper was to elucidate the effect of immobilization on collagen transformation of lateral collateral ligament(LCL) in the knees of rats. Methods Five Wistar rats underwent intraperitoneally the injection of 3H-proline, three times/per week, for 6 weeks.After four weeks,the right knees of rats were immobilized by internal fixation in flexion for 8 weeks.CPM of labeledcollagen of LCL was measured with liquid scintillation spectromery.Collagen mass of LCL was determinedby aminoacid analyzer system. Results The CPM value and collagen mass of immobilizedLCL in rats were significantly decreased, as compared with the those of contralateral collateralligament. There was obvious difference in both sides(P<0.05). New gain collagen of LCL was 32.35%,loss of old collagen was 42.41%. The net loss of collagen was 10.05%. ConclusionThese results demonstrate that the rate of degradation of collagen is considerably higher than the rate of synthesis of collagen after the knee immobilization of rats.