胸腺内注射异基因抗原诱导免疫耐受的实验研究

Study of inducing immunotolerance by intrathymical injection allogenic H-2 antigen

目的 :探讨胸腺内注射异基因抗原在建立特异性移植免疫耐受中的作用。方法 :自供体C5 7BL 6小鼠脾细胞中提取的H 2抗原 ,注入受体Balb c小鼠胸腺内 ,1w后移植供体C5 7BL 6小鼠皮肤及无关供体C3H小鼠皮肤。观察皮肤存活时间 ,同时做单向混合淋巴细胞培养 (mixedlymphocyteculture,MLC)、细胞介导的淋巴细胞毒 (cellmediatelymphocytotoxic ,CML)、迟发型超敏反应 (delayedtapehypersensitivity ,DTH)的检测。结果 :实验组移植皮肤平均存活时间 (mediansurvivaltimes,MST) >70d ,对照组MST为 12 .6± 1.6 9d ,移植C3H皮肤的无关供体组MST为 13.4± 1.42d。耐受小鼠淋巴细胞对供体淋巴细胞刺激的反应性减弱 ,而对无关供体淋巴细胞的刺激呈正常反应 ,耐受小鼠对供体靶细胞的CML作用、DTH反应特异性降低。显示出胸腺内注射抗原诱导耐受的特异性。结论 :胸腺注射异基因H

Objective:To study the effects of intrathymical injection allogenic antigen on the establishment of donor specific transplantation immunotolerance.Methods:H 2 antigen extracted from splenic cells of donor C56BL/6 mice was intrathymically injected to recipients adult Balb/c mice, one week later,donor C57BL/6 or C3H skingrafts were transplanted,observing transplanted skin survival times ,meanwhile MLC?CML ?DTH was detected.Results:the median survival time (MST) of transplanted skin allografts was over 70 days in tolerance recipients while the skin MST graft in the control group was 12.6±1.69 days,tolerance recipients normally rejected the donor C3H skin grafts (MST 13.4 ± 1.42 days), MLC detection showed that tolerance recipient splenocyte responded to C57BL/6 stimulator significant reduction ,but in normal reaction to donor C3H stimulator,the CML and DTH of tolerance recipient splenocyte to target cell of donor C57BL/6 was inhabited.The results suggested that slpecific tolerance might be induced by intrathymic injection. Conclusion:The intrathymic injection of donor H 2 antigen extracted from C57BL/6 splenic cells could induce recipients specific tolerance to skin transplantation.