乳腺癌中细胞周期素D1基因扩增和过表达及其与c-erbB-2的关系

Amplification and overexpression of cyclin D1 gene in breast cancer and their relations to c-erbB-2 protein

目的 探讨细胞周期素D1基因和表达的改变在乳腺癌中的作用及其与c erbB 2表达的关系。方法 采用原位分子杂交和免疫组织化学技术检测 5 9例乳腺癌组织细胞周期素D1基因扩增、mRNA及其蛋白和c erbB 2的表达。结果 2 7例 (4 5 8% )细胞周期素D1蛋白过表达 ,其过表达与腋淋巴结转移相关 (P <0 0 5 )。细胞周期素D1DNA扩增、mRNA高表达和蛋白过表达三者基本一致 ,与c erbB 2过表达无相关。结论 细胞周期素D1蛋白过表达在乳腺癌频发 ,其基因扩增和转录增加是其重要机制之一。细胞周期素D1和c erbB 2在乳腺癌中各自发挥独立作用 。

Objective To investigate the role of the alteration of cyclin D1 gene and its expression in human breast cancer and their relations to c erbB 2 oncoprotein. Methods Cyclin D1 DNA amplification and its mRNA and protein overexpresion in 59 cases of breast cancer tissues were detected by in situ hybridization and immunohictochemical staining. C erbB 2 oncoprotein expression was detected by immunohictochemical technique, too. Result Cyclin D1 was overexpressed in 27 cases of breast cancer (45 8%), which was significantly related to lymphnode involvement ( P <0 05). Coherence was found between cyclin D1 gene amplification, its mRNA and protein overexpression. Cyclin D1 overexpression was not associated with c erbB 2 positivity. Conclusion There are frequent alterations of cyclin D1 in human breast cancer. One of the mechanisms of cyclin D1 overexpression is its DNA amplification. Cyclin D1 and c erbB 2 may play important roles independently in the development of breast cancer through different pathways. Simultaneous detection of the two proteins is helpful to evaluate prognosis of the patients.