水蛭、丹参及其复方对血瘀大鼠血管内皮细胞保护作用的研究

Study of Protective Effect of Leech, Radix Salviae Miltiorrhizae and its Composite Recipe on Vascular Endothelial Cells in Rats with Blood Stasis Syndrome

目的 :研究活血化瘀中药 ,保护血管内皮细胞 (VEC)的作用 ,进一步为探讨血瘀证及活血化瘀机理 ,提供新思路和途径。方法 :用注射肾上腺素 ,并附加冰浴的方法 ,复制血管内皮损伤的血瘀模型 ,检测血循环内皮细胞 (CEC)数和血液流变学 ,并观察灌服大鼠水蛭或丹参及其复方 5d或 10d后的变化。结果 :该血瘀模型 ,具有高度重复性的CEC数增加 ,与全血粘度、纤维蛋白原和红细胞压积增加相并行。服中药的各组 ,CEC数明显减少及血液流变学的改善。结论 :该血瘀模型有明显的血管内皮损伤 ,水蛭。

Objective:To study the protective effect for Chinese medicine of promoting blood circulation and removing blood stasis (PBCRBS) on vascular endothelial cells (VEC) in rats,aimingatfurther research on the mechanism of blood stasis syndrome and PBCRBS.Method:Establishing a model of blood stasis with endothelial damage by means of giving rats an injection of adrenalin and making it swim in ice-cold water,then measuring the number of circulating endothelial cells (CEC) in whole blood and rheology. Moreover observing the change of the above indexes after the rats have taken Leech?Radix salviae miltrorrhizae (RSM) and its composite recipe for 5 days or 10 days.Result:It could increase the number of CEC accompanied by whole blood viscosity, fibrinogen and hematocrit in rats with blood stasis.But for groups taking Chinese medicine, the number of CEC was decreased distinctively and blood rheology was improved. Conclusion:The rat with blood stasis syndrome suffered from obvious injury of VEC. The Leech,RSM and its compositerecipe could protect VEC.