白附子对人T细胞和单核细胞的调节活性

Human T Cell and Monocyte Modulating Activity of Rhizoma Typhonii in Vitro

目的 :研究中药白附子的免疫学调节活性。方法 :用3H介入法测定了中药白附子提取物(Rhizomatyphoniiextract,RTE)对小鼠脾细胞和人淋巴细胞的增殖活性 ;用绵羊红细胞花结形成试验分离纯化淋巴细胞后 ,检测了RTE的效应细胞 ;用51Cr游离实验测定了人杀伤细胞和自然杀伤细胞的杀伤活性 ,用ELISA法和生物法研究了单核细胞的细胞因子产生 ,并用3H介入法分析了单核细胞对肿瘤细胞的吞噬功能 ;用层析法分析了白附子的化学成分 ,并用SDS PAGE法测定了其有效成分的分子量。结果 :RTE对小鼠脾细胞和人淋巴细胞的增殖活性有很强的促进作用 ,并有较好的量效关系 ;RTE的效应细胞是T细胞 ;RTE增强人杀伤细胞对肿瘤细胞的特异性杀伤活性和自然杀伤细胞的非特异性杀伤活性、增强单核细胞的细胞因子 (肿瘤坏死因子和白细胞介素 1)产生 ,并增强单核细胞对肿瘤细胞的吞噬功能。白附子的化学成分为 66道尔顿的糖蛋白。结论 :RTE对人的T细胞和单核细胞有免疫增强作用 ,并通过刺激机体的免疫系统杀伤或吞噬肿瘤细胞和外来抗原 ,这些活性可应用于临床调节免疫功能和治疗肿瘤等疾病。

To study the immunological modulating activity of Rhizoma typhonii (RT) on lymphocytes in vitro. Methods: Effect of RT extract (RTE) on mice spleen cells and human lymphocytes proliferation was detected by sheep erythrocytes rosette forming test after lymphocytes had been isolated and purified. The activity of human killer cells and natrral killer (NK) cells was tested using 51 Cr free test. The production of cytokine of macrophages was examined using ELISA test and biological test and the phagocytic function of macrophage on tumor cell was determined using 3?H incorporation assay. The chemical ingredients of RT were analyzed using chromatography and the molecular weight of their active principle was determined using sodium dodecyl sulfate polyacrylamide gel electrophoresis technique (SDS PAGE) method. Results: RTE could markedly stimulate the mice spleen cells and human lymphocytes to proliferate in a dose effect dependent manner; the effector cell of RTE is T cells. RTE enhanced human killer cell, the allo antigen specific cytotoxic T lymphocytes (CTL) activity and the nonspecific activity of NK cells. Furthermore, RTE stimulated macrophages to produce tumor necrosis factor (TNF α) and interleukin 1 (IL 1), and enhanced phagocytic activity of macrophage to tumor cells. The elution of RTE from chromatography showed that the active principle of RT was glycoproteins in nature and had an apparent molecular weight of 66kDa. Conclusion: RTE has immunoenhancing activity to human T cell and macrophage, through stimulating the killer cell and phagocytosis of tumor cell and allo antigen, which could be used clinically for modulating immune responses and for treating tumor and other diseases.