慢性乙型肝炎患者血清TNF-α、LN、HA、IV-C的检测及其临床意义

The Serum levels and their clinical significances of tumor necrosis factor-α,laminin,hyaluronate and type-IV collagen in patiants with chronic hepatits B

目的 探讨慢性乙型肝炎 (以下简称乙肝 )患者血清肿瘤坏死因子 (TNF α)、层粘蛋白 (LN)、透明质酸(HA)、IV型胶原 (IV C)的变化规律及临床意义。方法 血清TNF α采用放射免疫法测定 ;LN、HA及IV C采用酶联免疫法测定。结果 4项指标均随着病情的加重而逐步升高 ,在慢性乙肝重度及肝硬化阶段达高峰 ,与肝纤维化程度一致。TNF α在慢性乙肝重度时升高幅度最大 ,TNF α的变化与LN、HA及IV C的改变呈显著正相关关系(P <0 .0 1)。结论 联合检测血清TNF α、LN、HA及IV C是较好反应肝纤维化程度和肝内炎症变化的有效指标。

Objective To investigate the seria changes and their clinical significances of tumor necrosis factor α(TNF α), laminin (LN), hyaluronate (HA) and type IV collagen (IV C) in patient with chronic B.Methods serum levels of TNF α were determined by the method of radioimmunological assay. LN, HA and IV C levels were determined by the method of ELISA.Results The serum levels of TNF a, LN,HA and IV C increased according to severity of hepatic damange in patient with chronic hepatits B and raised to their highest levels in the patients with liver cirrhosis and severe type of chornic hepatitis .We also found rise of these four markers was consistent with the degree of hepatic fibrosis.The correlations of TNF α and LN,HA and IV C were statistically significant (P<0.01).Conclusions The serum levels and their serial changes of TNF α and LN. IV C in patients chronic hepatitis B may be useful indicators to reflect the severity of hepatic damage and hepatic fibrosis.