急性特发性血小板减少性紫癜免疫功能的观察

Observation of immunologic status in children with acute idiopathic thrombocytopenic purpura.

目的 :探讨急性特发性血小板减少性紫 (ITP)患儿细胞因子 :血清可溶性白介素 - 2受体 (sIL - 2R)、白介素 - 6(IL - 6) ,免疫球蛋白轻链 ,心磷脂抗体 (ACA)及淋巴细胞亚群的变化。方法 :应用化学发光法 ,速率散射比浊法 ,ELISA法及AKP -ABC法。结果 :急性ITP患儿治疗前sIL - 2R、IL - 6升高 ,轻链κλ降低与正常对照P <0 0 1,3个月后恢复至对照组近似水平 (P均 >0 0 5 )。ACA阳性率占 70 3 7%。CD+4细胞减少 ,CD+8细胞增多 ,CD+4/CD+8比值下降 (P均 <0 0 1)。结论 :为临床提供判断疗效及估计预后较血小板 (BPC)计数更可靠的依据 。

Objective:To explore changes of cytokines in children with acute idiopathic thrombocytopenic purpura,serum soluble interleukin-2 receptor(sIL-2R),interleukin-6(IL-6),light chain of immunoglobulin,ACA and subgroups of lymphocyte were measured.Methods:These factors were detected by chemiluminance manner,rate scatter turbidimetry,ELISA and AKP-ABC respectively.Results:Before treatment SIL-2R and IL-6 in children with ITP were decreased,while κ?λ light chain of imunoglobulin were increased when compared with normal controls (P<0.01).After 3 treatment,there were no difference between two groups (P>0.05).Positive rate of ACA was 70.37%.CD + 4 cells decreased significantly,while CD + 8 cells increased, CD + 4/CD + 8 was lower in patients group than in control group (P<0.01).Conclusios:The determination of these immunologic factors was important in studying effect of treatment and estimating the patients' prognosis.These results have laid the fundation for further study of the application of immunomodulator in ITP.