软肝化癥丸抗肝纤维化作用的实验研究

Exper imental Study on Anti-hepatofibrosis Acttivity of RuanGanHuaZhengWan(RGHZW)

目的 :探讨多法联用经验方软肝化丸对肝纤维化的治疗作用及作用机理。方法 :复制大鼠免疫性肝纤维化模型 ,取大鼠肝组织及血清观察肝脏和血清生化、血液流变学、肝脏病理、胶原基因表达、脂多糖诱导腹腔巨噬细胞产生细胞因子。结果 :软肝化丸具有保护肝功能 ,改善血液流变性 ,降低肝组织胶原蛋白的含量 ,抑制诱导的大鼠腹腔巨噬细胞产生一氧化氮 (NO)水平、肿瘤坏死因子a(TNF a)和白介素 1(IL 1)的活性 ,抑制大鼠肝组织Ⅰ型胶原和Ⅲ型前胶原mRNA的表达。结论 :软肝化丸能够保护大鼠肝功能 ,通过调节免疫及肝组织胶原蛋白的过度增生与沉积而达到抗肝纤维化的目的。

To study the anti hepatofibrosis activity and mechanism of RGHZW, as an effective prescription which contains multi therapies for the treatment of hepatic fibrosis as a result of chronic liver diseases. Used procine serum to induce hepatic fibrosis rat model. To take model of liver and serum to observe biochemic change, the indexes of blood rheology, pathology of liver, cytokine produced by the macrophage in abdominal cavity, and genic expression of collagen. The results showed RGHZW in different dosage obviously lowered serum level of ALT、AST in liver tissue, and improved the albumin in serum, and reduced the content of Hyp and the hyperplasia of collagen in the liver tissue, and improved the indexes of blood rheology, and alleviated the denaturation and necrosis of hepatocytes; and restrained NO、TNF α、IL 1 induced by macrophage in abdominal cavity; and significantly depressed the mRNA expression of type Ⅰ and type Ⅲ collagen in fibrotic rat liver. These results suggested that RGHZW protected the liver function and regulated the function of immunity in fibrotic rat,. The proliferation and aggradation of collagenous fibers were restrained. These results showed better antihepatofibrosis effect in experimental rats.