质谱在肽和蛋白质序列分析中的应用

Application of Mass Spectrometry on the Sequencing of Peptides and Proteins

了解肽和蛋白质的序列对理解其功能具有重要意义 ,测定其序列也是当前生命科学研究中的重要内容之一 .质谱作为高灵敏度的测定分子结构的仪器 ,其高灵敏度、广泛的适用性及快速性等特性使它具有很大潜力发展成为辅助传统测序方法的新方法 ,并得到了广泛的关注 .从离子活化方法 (包括碰撞诱导解离CID、源后裂解PSD、源内裂解ISD等 )、衍生化作用以及氨基酸残基消除方式 (高能活化产生亚稳离子、化学降解、酶降解 )等多个角度介绍了利用质谱分析多肽和蛋白质序列的方法 。

Efficient and automatic methods of sequencing peptides and proteins are always the most demanded techniques in biological research. As a promising tool on the elucidation of molecular structures, mass spectrometry has been used in the sequencing of peptides and proteins for several years. It has become more and more popular in the biological work. This paper reviews the different methods on this technique, which include the activation methods ( collision-activated dissociation, postsource decay, irisource decay, et al.), derivative influence and so on.