多巴胺和5-羟色胺的毛细管电泳测定

Analysis of 5 -Hydroxytryptamine and Dopamine by Capillary Electrophoresis

建立了高效毛细管电泳结合柱上紫外检测测定单胺类神经递质多巴胺(dopamine,DA)和5_羟色胺(5_hydroxytryptamine,5_HT)的毛细管电泳方法 ,优化了缓冲液 pH值、浓度、电压等实验参数 ;结果表明 ,在优化的实验条件下 ,多巴胺、3,4_二羟苄胺 (3,4_dihydroxybenzylamine)和5_羟色胺在10min内得到很好的分离 ,检出限分别为2.02×10 -5mol/L,1.01×10 -5mol/L,1.20×10 -5mol/L ;该法简便、快速 ,样品用量少 ,结果准确 。

A method for separation of dopamine and 5 _ hydroxytryptamine by capillary electrophoresis with ultraviolet detection is described. The effects of pH value and concentration of running buffer, and an applied voltage were investigated in detail. The results showed that dopamine, 3,4 _ dihydroxybenzylamine and 5 _ hydroxy_tryptamine were eluted in 10 min with satisfactory separation and the detection limits were 2.02×10-5 mol/L, 1.01×10-5 mol/L and 1.20×10-5 mol/L, respectively. The method is simple, rapid, accurate and repeatable.