复方维生素B片中主要成分的高效毛细管电泳电化学法检测

Determination of Main Components in Vitamin B Tablet byCapillary Electrophoresis-Electrochemical Detection

采用高效毛细管电泳 -电化学检测法同时测定复方维生素B片中的主要成分维生素B1、B12、B6 和C的含量 ;研究了电极电位、运行缓冲溶液的浓度和酸度、电泳电压和进样时间等对电泳的影响 ;以微铂电极为工作电极 ,检测电位 +0.5V(vsSCE),在pH9.0的15mmol/LTris-1mmol/LH3BO3 缓冲溶液中 ,上述4组分在5min内获得基线分离 ;维生素B1、B12、B6 和C的线性范围分别为2.1mg/L～1.0g/L、6.0mg/L～0.80g/L、1.4mg/L～0.72g/L和0.97mg/L～0.44g/L;检出限分别为0.50mg/L、1.0mg/L、0.65mg/L和0.40mg/L;5次测定峰高的相对标准偏差分别为2.4%、3.0 %、3.1 %和2.5 %;5次测定的平均回收率分别为99 %、102 %、98 %和100 %。

The separation and detection of main components vitamin B1, B12, B6 and C in compound vitamin B tablet are reported by home _ made capillary electrophoresis apparatus with electrochemical detector. The effects of working electrode potential, concentration and pH value of running buffer, separation voltage and injection time on the CE-ED determination were investigated. A platinum electrode with diameter of 300 μm was used as the working electrode at potential of +0.5 V. Vitamin B1, B6, B12 and C could be well separated in 15 mmol/L Tris-1 mmol/L H3BO3 buffer at pH 9.0 within 5 min. The linearity between peak current and concentration of VB1, VB2, VB6 and VC existed in the range of 2.1 mg/L～1.0 g/L, 6.0 mg/L～0.80 g/L, 1.4 mg/L～0.72 g/L and 0.97 mg/L～0.44 g/L, respectively. The detection limits of 0.50 mg/L, 1.0 mg/L, 0.65 mg/L, 0.40 mg/L, and RSD(n=5) of 2.4%, 3.0%, 3.1%, 2.5% and average recovery(n=5) of 99%, 102%,98%, 100% were obtained for VB1, VB12, VB6 and VC, respectively.