散利痛中扑热息痛和异丙基安替比林的毛细管区带电泳电化学检测法测定

Determination of Paracetamol and Isopropylphenazone in Compound Propyphenazoni Tablets by Capillary Zone Electrophoresis with Electrochemical Detection

毛细管区带电泳 -电化学检测法同时测定散利痛片中有效成分扑热息痛和异丙基安替比林的含量 ;研究了电极电位、电解液浓度和酸度、电泳电压及进样时间等对电泳的影响 ,得到了最优化的测定条件 ;以直径为300μm的碳圆盘电极为检测电极 ,工作电极电位为1.0V(vsSCE) ,在50mmol/L硼砂 -NaOH(pH9.35)运行缓冲液中 ,上述两组分在8min内完全分离 ;扑热息痛和异丙基安替比林线性范围分别为2×10-3～5×10 -6mol/L和2×10 -3～2×10 -6mol/L,检出限分别为5×10 -6mol/L和2×10 -6mol/L ;7次平行进样的相对标准偏差(RSD)为3.5%和1.8 % ,加标回收率(n=3)分别为101 %和98% ,该法灵敏可靠 ,结果令人满意。

A method for the simultaneous determination of paracetamol and isopropylphenazone in compound propyphenazoni tablets is reported by capillary zone electrophoresis with electrochemical detection(CE-ED). The effects of working electrode potential, concentration and pH of the running buffer, separation voltage and injection time on the determination were investigated and discussed. Paracetamol and isopropylphenazone could be well separated in a 50 mmol/L borax-NaOH buffer at pH 9.35 within 8 min. There is excellent linearity between peak current and analyte concentration in the range of 2×10-3～5×10-6 mol/L for paracetamol and 2×10-3～2×10-6 mol/L for isopropylphenazone with the detection limits(S/N=3) of 5×10-6 and 2×10-6 mol/L for paracetamol and isopropylphenazone, respectively. Relative standard deviations of the peak currents of paracetamol and isopropylphenazone were 3.5% and 1.8% (n=7), respectively. Recoveries of 101% for paracetamol and 98% for isopropylphenazone were obtained (n=5).