高效毛细管电泳-电导检测拆分联萘酚对映体

Chiral Separation of Binaphthol Enantiomers by Capillary Electrophoresis Combined with Conductance Detection

以 Na OH和β-CD作为运行液 ,采用高效毛细管电泳 -电导检测对联萘酚进行了拆分研究 ,考察了影响分离和检测效果的实验参数 ,并对其拆分机理进行了讨论和验证 .结果表明 ,在选择的最佳实验条件下 ,联萘酚对映体在 1 5 min内得到基线分离 .

The binaphthol enantiomers were resolved by capillary electrophoresis combined with conductance detection. The β cyclodextrin( β CD) was used as a chiral reagent in sodium hydroxide solution. The Pt wire with diameter of 80 μm was used as working electrode. The separation parameters, such as concentration of running buffer and β CD, applied voltage, have been studied in detail. Under the optimum conditions, baseline separation of the enantiomers could be completed in 15 minutes . The separation mechanism has been studied by using methylated β CD as a chiral reagent. It was found that the H bonding is an important factor determining the interaction between molecules.