固定化青霉素酰化酶的新载体

A New Carrier for immobilization of penicillin acylase

通过X射线粉末衍射、红外光谱等手段考察了α 磷酸锆层柱材料作为载体固定青霉素酰化酶及其对载体结构影响。结果表明无论是否加入戊二醛固定化酶后 ,层柱材料的结晶度都下降。酶活性和回收率较高 ,分别为139 5nmol/s和 2 8 9%。间歇操作稳定性较好 ,连续操作 10次 ,酶活性下降 2 4%

Penicillin acylase immobilized on triethylaminetetramine intercalacated α ZrP was prepared and characterized by the techniques of X ray powder diffraction and infrared spectrophotometry.Immobilization of the enzyme led to a decrease in crystallinity of the host solid whether there was glutaraldehyde. The activity of the immobilized enzyme was 139 5 nmol/s, and decreased by only 24% after reusing 10 times.