乙氧苯柳胺-2-葡萄糖苷酸的分离鉴定

Isolation and Identification of Etofesalamide-2-glucuronide

报道了治疗痤疮新药乙氧苯柳胺 [N-(4 -乙氧苯基 ) -2 -羟基苯甲酰胺 ]的葡萄糖苷酸结合物的制备方法 .选择 2只健康家兔 ,每日口服 40 0 mg乙氧苯柳胺 ,收集 0～ 1 2 h尿样 ,尿样经冷冻干燥 ,甲醇提取后 ,以半制备型液相色谱对粗提物中乙氧苯柳胺 -2 -葡萄糖苷酸进行了分离制备 ,并采用电喷雾离子阱质谱法与1 H

Preparation of glucuronide conjugates of etofesalamide\[\%N\%\|(4\|ethoxyphenyl)\|2\|hydroxyl\|benzamide\], a novel drug for acne therapy, in rabbit was discribed. Urine samples from 0 to 12 h were collected after single oral administration of 400 mg etofesalamide to two rabbits. The samples were lyophilized and extracted with methanol, and then etofesalamide\|2\|glucuronide in them was prepared with semi\|preparative HPLC. The molecular structure of this compound was confirmed by electrospray ion trap mass spectrometry and \{\}\+1H NMR.