多重PCR-核酸探针杂交法同时检测沙眼衣原体、解脲支原体和淋病奈瑟菌

Simultaneous detection of Chlamydia trachomatis,Ureaplasma urealyticum and Neisseria gonorrhoeae by multiplex PCR-liquid DNA hybridization

目的 建立一种同时检测沙眼衣原体、解脲支原体、淋病奈瑟菌三种病原体DNA的方法并用于临床标本检测。方法 采用多重PCR 核酸探针杂交技术 ,结果与常规PCR法比较。结果 本法特异性良好 ,敏感度达到 0 1fg。经过对 166例性传播疾病门诊患者标本测定证实 ,沙眼衣原体、解脲支原体、淋病奈瑟菌检出率分别为 2 2 9% ,5 4 8%和 3 4 3 % ,并有 2 2 2 %的双重复合感染和 4 8%的三重复合感染。并与常规PCR法具有很好的一致性。结论 本法快速、简便、特异性好、能指示多重感染。

Objective To develop a method for simultaneous detection of DNA of CT,UU and NG in clinical specimens.Method A multiplex PCR and target specific oligonucleotides probes for hybridization in a microwell format with colorimetric detection was designed and compared with the routine PCR method.Results Laboratory tests showed that this method has a high specificity and sensitivity(0 1 fg).166 swab specimens collected from STD patients Luoyang in 1998 were derected.The positire rates of CT,UU and NG were 22.9%,54.8% and 34 3% respectively meanwhile,the co infection of two and three etiologic agents were 22 2% and 4 8% respectively.It is concordant with routine PCR method.Conclusion The results indicate that the multiplex PCR assay is a simple,rapid,specific and sensitive method for detection of the three etiologic agents simultaneously.