摘要
细胞色素氧化酶 (CytochromeOxidase ,CO)是呼吸链上的最后一个酶 ,是细胞能量产生和传递的关键组成部分。在中枢神经系统中 ,区域性CO活性反映了局部组织能量代谢变化 ,这种变化与神经元的功能尤其是神经元突触和自发性电活动的能量需求密切相关。CO的组织化学染色方法已被广泛用于反映中枢神经系统CO的活性。为了成功地进行CO的组织化学染色 ,适中的组织固定是关键。固定剂的组成必须考虑既要保持良好的组织结构又要保持较高的酶活性。通常使用多聚甲醛和戊二醛混合固定液。本实验选用不同浓度的多聚甲醛和戊二醛固定组织 ,做CO组织化学染色 ,并进行光镜和电镜观察 ,发现 1%多聚甲醛和 2 %戊二醛混合固定既可保存良好的组织结构又可获得较高的CO活性。
Cytochrome oxidase (CO) is a terminal enzyme of mitochondrial respiratory chain and a vital component of cellular energy production and transduction. In the central nervous system, regional CO activity provides an index for local tissue energy metabolism, which is closely coupled to neuronal depolarizing activities, in particular of synaptic and spontaneous types. CO histochemistry has been widely used to demonstrate CO activity. For successful CO histochemistry, proper fixation is crucial. Compromise has to be made between the quality of tissue fixation and the preservation of enzyme activity. Usually, a mixture of paraformaldehyde and glutaraldehyde is used. The present study was aimed to work out the best combination of these two fixatives for both light and electron microscopic CO histochemistry. It was found that a mixture of 1% paraformaldehyde and 2% glutaraldehyde serves the purpose best in the areas of the central nervous system of the adult rat we investigated. \[
基金
国家自然科学基金 (3 9870 2 64 )资助项目
