斑秃患者血T细胞变化及其凋亡信号控制

Regulation on apoptosis messages and changes on activated T-lymphocytes in patients with alopecia areata

目的 探讨活化T淋巴细胞在斑秃发病中所起的作用 ,并研究bcl 2和CD95在T淋巴细胞上的表达对斑秃的发生、发展所造成的影响。方法 先用淋巴细胞分离液提取淋巴细胞 ,再用流式细胞仪分别检测CD4 + T细胞、CD8+ T细胞、CD95及bcl 2在T细胞中的表达。结果 在斑秃处于活动期时 ,CD4 + T细胞明显减少 ,而CD8+ T细胞明显增多 ;在同时期 ,CD4 + bcl 2表达减少 ,CD8+ bcl 2则表达增多。CD95在T细胞上的表达也显著增加。结论 CD4 + T细胞因其bcl 2表达减少而减少 ,CD8+ T细胞则因其bcl 2表达增加而增加 ,导致CD4 + /CD8+ 比值大幅度降低 ,CD8+ T细胞对毛囊细胞的杀伤作用加强了。同时 ,CD95在T细胞上的表达增加 ,通过另一条途径加快了毛囊细胞的凋亡。

Objective To investigate the role of activated T lymphocytes in the pathogenesis and effect of CD95 and bcl 2 expression on the development of alopecia areata. Methods The lymphocytes were isolated from the peripheral blood of the patients. Then CD4 +, CD8 +,and/or CD95 and bcl 2 positive lymphocytes were sorted out by flow cytometry . Results In the active stage of the disease, CD4 + lymphocytes decreased while CD8 + lymphocytes increased significantly. At the same time, the expression of bcl 2 was much more frequently detected in CD8 + lymphocytes than in CD4 +lymphocytes. CD95 expression was also increased markedly in T lymphocytes. Conclusions Down regulation of bcl 2 leads to the decrease in number of CD4 + lymphocytes. Meanwhile, CD8 + lymphocytes increased duo to the over expression of bcl 2. As a result , the ratio of CD4 + to CD8 +lymphocytes has dropped markedly and the cytotoxic effect of CD8 +lymphocytes on the hair follicles is enhanced. On the other hand, over expression of CD95 in T lymphocytes triggers cell apoptosis in the hair follicles. Both effects promote the development of alopecia areata . [