苦参碱对人增生性瘢痕成纤维细胞细胞周期和DNA含量的影响

Influence of matrine on the cell cycle and DNA content of fibroblasts derived from hypertrophic scar

目的 研究苦参碱对人增生性瘢痕成纤维细胞细胞周期和DNA含量的影响。方法 将不同浓度梯度苦参碱作用于体外培养的增生性瘢痕成纤维细胞 ,计算其群体倍增时间 ,通过流式细胞仪检测其细胞周期、DNA相对含量。结果 不同浓度梯度的苦参碱作用增生性瘢痕成纤维细胞 ,其群体倍增时间与苦参碱浓度呈正相关 ;随着苦参碱作用细胞的时间逐渐延长 ,出现G0 /G1期数量增加 ,S期、G2 M细胞数量逐渐减少 ,DNA相对含量无明显变化。结论 苦参碱能明显抑制增生性瘢痕成纤维细胞的有丝分裂 。

Objective To study the effect of matrine on the cell cycle and DNA content of fibroblasts derived from hypertrophic scarring tissues. Methods The fibroblasts were derived from 3～5 generations of the cultured hypertrophic scarring tissues. After the cells were treated with different concentration of matrine, the population doubling time was calculated and the cell cycle and DNA content were measured with flow cytometry. The fibroblasts treated with normal saline were as control. Results Compared with the control group, the distributions in cell cycle showed that the percentage of G 2 M and S phase cells decreased, and the percentage of G 0 G 1 phase cells increased significantly ( P <0.01).The contents of DNA were stable during the treatment. Conclusion Matrine can significantly block the mitosis of fibroblasts derived from hyperotrphic scarring tissues, but does not change the contents of DNA of the cells.