α-干扰素治疗慢性乙型肝炎患者血清肿瘤坏死因子-α和白细胞介素-8水平与抗病毒应答的关系

Relationship between tumor necrosis factor-α and interleukin-8 level in serum in patients with chronic hepatitis B and response to interferon-α therapy

目的 :观察慢性乙型肝炎患者经α 干扰素 (IFN α)抗病毒治疗后血清中肿瘤坏死因子 α(TNF α)和白细胞介素 8(IL 8)水平的变化与乙型肝炎病毒消长之间的关系。方法 :收集治疗前后血清标本 - 2 0℃保存 ,通过ELISA法同步检测并与正常人做对照。结果 :IFN α应答组 (n =10 )在治疗前血清中TNF α水平明显低于非应答组 (n =15 )和正常组 (n =10 ) ,P <0 0 1;但IFN α治疗后无论是应答组还是非应答组均较治疗前下降 ,尤其是非应答组下降更明显。所有慢性乙型肝炎病例血清中IL 8水平均较正常组低 ,经IFN α治疗后均较治疗前升高并接近正常人水平 ,但应答组和非应答组之间比较无显著差异。结论 :慢性乙型肝炎患者体内存在免疫功能的失调 ,IFN α具有抑制TNF α和增进IL 8表达的调节作用 ,但体内病毒复制消长与TNF α和IL

AIM: To study the relationship between the changes of tumor necrosis factor α (TNF α) and interleukin 8 (IL 8) level in serum of patients with chronic hepatitis B (CHB) before and after interferon α (IFN α) treatment and expression of hepatitis B virus (HBV) marker. METHODS: Enzyme liked immunosorbent assay (ELISA) was performed in 25 patients with CHB to determine levels of TNF α and IL 8 in serum. RESULTS: The level of TNF α in patients' group (15) with response to IFN α before treatment decreased significantly as compared with no response group (10) or normal group (10) ( P <0 01), the TNF α level in both response and no response group after IFN α treatment was significantly decreased ( P <0 01), the TNF α level in no response group was significantly lower than that in response group. The serum IL 8 level in patients with CHB before IFN α treatment was significantly lower than normal subjects ( P <0 01), the IL 8 level in patients after IFN α treatment was increased, but no significant difference was found between response and no response group. CONCLUSION: The results suggest that IFN α can play an important role in positive regulation for IL 8 level in patients with CHB, but HBV replication could be not relative to TNF α and IL 8 levels in serum.