IL-1基因多态性与牙周炎关系的研究

The Association between IL-1 Gene Polymorphisms and Susceptibility to Severe Periodontitis

目的 :研究中国汉族人IL_1基因多态性与牙周炎易感性的关系。方法 :收集 30例重度成人牙周炎 (AP)患者、2 0例快速进展型牙周炎 (RPP)患者和 94例健康对照者的颊粘膜拭子 ,抽提DNA ,PCR_RELP方法检测IL_1基因簇基因多态性 ,比较三组患者各等位基因检出率的差异。结果 :AP组和RPP组IL_1B +395 3 TaqI等位基因Ⅱ的检出率均显著高于对照组 (AP 对照组OR =6 8,RPP 对照组OR =9 6 ,P <0 0 5 ) ,AP组IL_1RN内含子 2 VNTR等位基因Ⅱ的检出率也显著高于对照组 (OR =6 3,P <0 0 5 )。IL_1A_889 NcoI等位基因在三组间的分布无显著性差异(P >0 0 5 )。结论 :中国汉族人中携带IL_1B +395 3 TaqI等位基因Ⅱ和IL_1RN内含子 2 VNTR等位基因Ⅱ可能是牙周炎的遗传易感因素 ;

Objective: The purpose of this study was to investigate the relationship between IL_1 gene polymorphisms and susceptibility to severe periodontitis in Chinese of the Han Nationality.Methods: DNA samples with buccal swabs were collected from 30 patients with severe adult periodontitis (AP), 20 patients with rapid progressive periodontitis (RPP) and 94 healthy control (HC) individuals. Polymorphisms in the IL_1 gene cluster were analyzed with PCR_RFLP.Results: It was found that the frequency of IL_1B +3953/Taq I allele 2 was significantly higher in severe AP patients (OR=6.8, P<0.05) and RPP patients (OR=9.6, P<0.05) than in healthy controls, and the frequency of IL_1RN intron II/VNTR allele 2 was significantly higher in the group of severe AP patients (OR=6.3, P<0.05) than in the group of healthy controls.Conclusion: IL_1B +3953/Taq I allele 2 and IL_1RN intron II/VNTR allele 2 are genetic indicators of susceptibility to severe AP and RPP, and both AP and RPP are genetic heterogeneous.