乙型肝炎病毒核酸定量检测与临床的关系

Nucleic acid quantifying assay of hepatitis B virus

目的 探讨血清ＨＢＶ ＤＮＡ水平与ＨＢＶ标志物（ＨＢＶ Ｍ）表现摸式、吁功能状态、肝内炎症的关系。方法 对２１９例排除甲、丙、丁和戊型肝炎病毒的混合与重叠感染患者中的３９例ＨＢｓＡｇ阳性的慢性乙型肝炎患者进行肝穿刺病理检查。ＨＢＶ ＤＮＡ定量采用荧光定量ＰＣＲ分析系统，ＨＢＶ Ｍ采用ＥＬＩＳＡ法。结果 血清ＨＢＶ ＤＮＡ水平与ＨＢＶＭ表现模式有关，ＨＢｓＡｇ与ＨＢｅＡｇ的存在影响ＨＢＶ ＤＮＡ水平变化。在ＨＢｓＡｇ阳性患者中，ＨＢＶ ＤＮＡ水平与Ｓｃｈｅｕｅｒ分级无明显相关。血清谷丙转氨酶水平与ＨＢＶ ＤＮＡ水平无明显相关。结论ＨＢｅＡｇ和ＨＢＶ ＤＮＡ有明显的相关，抗－ＨＢｅ阳性者病毒未完全停止复制，只是复制水平降低，单抗ＨＢｓ阳性，单抗ＨＢＣ阳性、抗ＨＢｓ阳性和抗 ＨＢｃ阳性未检出 ＨＢＶ ＤＮＡ；肝内炎症活动程度与血清ＨＢＶ ＤＮＡ水平无明显关系。

Objective To explore the relation between the nucleic acid content of hepatitis B virus and hepatitis B virus markers, liver function and its pathological state. Methods Thirty-nine patients suffering from chronic hepatitis B with positive HBsAg were performed liver puncture for histopathological examination. Fluorescence ration PCR analysis was used for HBV DNA quantifying assessment. Hepatitis B virus markers were detected by ELISA. Results Serum HBV DNA content was related to hepatitis B virus markers. There was no correlation between HBV DNA level and the Scheuer grade in patients with positive HBsAg, and no obvious correlation between serum glutamic pyruvic transaminase level and HBV DNA content. Conclusions There is a significant correlation between HBeAg and HBV DNA. but not between liver inflammation stage and serum HBV DNA content.