摘要
目的研究病毒性心肌炎(VMC)小鼠肿瘤坏死因子-α(TNF-α)的动态变化及其在VMC小鼠发病中的意义。方法本实验用ELISA方法检测VMC小鼠在接种病毒后3、5、7、9、15、35天血清TNF-α的变化,并分析了TNF-α与VMC发病的可能关系。结果实验发现VMC小鼠在接种病毒后3～35天各时点的血清TNF-α水平均明显高于对照组(P<0.05或0.01)。其表达高峰在接种病毒后7天(与对照组比较P<0.01)。结论结果提示小鼠VMC血清TNF-α水平升高可能参与VMC的发病。
Objective To study the change of serum tumor necrosis factor- a (TNF- a) in viral myocarditis (VMC) and its role in VMC. Methods Our experiment studied the change serum TNF- a on day 3, 5, 7, 9 , 15, 35 after virus inoculation in VMC mice by the way of ELISA, and analysed possible relation of TNF- a with VMC. Results The experiment found that serum TNF - a in VMC mice was higher than control mice at 3 - 35 days after virus inoculation, the expression peak was on day 7 after inoculation (P < 0.01 compared with control group) . conclusions The results showed that serum TNF- a in murine VMC increased, it may take part in the pathogenesis of VMC.
出处
《医学研究通讯》
2002年第1期38-39,共2页
Bulletin of Medical Research
