营养生态条件对蜜蜂球囊菌生长及产孢的影响

Effects on the mycelia growth and spore-forming of Ascosphaera apis under ecological condition of nutrients.

从碳源、氮源、维生素、矿质元素等方面研究了营养生态条件对蜜蜂球囊菌(Ascosphaera apis Olive＆Spiltoir)生长及产孢的影响。结果表明,营养生态条件的变化对蜜蜂球囊菌的影响极大。A.apis菌丝体生长最好的碳源是果糖,产孢量最高的是葡萄糖,不利用山梨糖和甘露糖;该菌优先利用有机氮源,不利用无机氮源中NaNO_2、H_2NCSNH_2;维生素对A.apis产孢量有极为显著的影响,复合维生素比单一维生素能更好地促进菌丝体生长,尤其对产孢更为有利;矿质元素中Mg^(2+)、P^(5+)、K^+、Zn^(2+)促进菌丝体生长和孢子的形成,Na^+对菌丝体的生长有抑制作用。

The effects on the mycelia growth and spore-forming of A. apis under ecological condition of nutrients -carbon and nitrogen sourse, vitamin and mineral elements are analyzed. The results show that the fungus grow best in D-fructose, spore-forming best in D-glucose but no growth occurs in D-mannose and D-sorbose. Except NaNO2 and H2NCSNH2,the fungus give priority to utilize organic nitrogen sources. Vitamins have distinctive promoting effect on the spore-forming of A. apis, and the effect of mixed application of several vitamins is more efficient than any single one. Mg2+,P5+,K+ and Zn2+ can promote the spore-forming and growth of the fungus, Na+ constrains the growth of the mycelia of A. apis.