原生质体非对称电融合获得不结球白菜胞质杂种

Synthesis of Cytoplasm Hybrid of Non-heading Chinese Cabbage through Asymmetric Electric Fusion of Protoplast Cell

以不结球白菜OguCMS‘91H秋 10 0’ (不育系 )和‘91H秋 2 1’ (保持系 )为试材 ,研究了不同交流场强、交流频率、直流脉冲场强、直流幅宽及脉冲次数对非对称细胞融合效果的影响。结果表明 ,不结球白菜电融合的最适电场条件为交流场强 2 0V/cm、交流频率15 0 0kHz、直流场强 2 0 0V/cm、直流脉冲幅宽 4 0 μs、直流脉冲次数 3次。融合产物用KM8P1培养基进行包埋培养 ,获得了 383块小愈伤组织 ,其中 32块分化出芽 ,形成 2 0株完整植株 ,驯化后栽培成活 8株 ,ZS2 、ZS6 、ZS8等 3株不育 ,后用保持系花粉授粉 ,分别收获种子。对胞质杂种的分子。

The effects of different electric fields on the asymmetrical cell fusion of non heading Chinese cabbage was studied by using OguCMS 91 Hqiu 100(sterile line) and 91 Hqiu 21 (maintainer line) as materials. The best electric field for electric fusion was as follows: alternating current field intensity (20V/cm), alternating current frequency (1500kHz), direct current intensity (200V/cm), direct current pulse width (40μs), direct pulse number (3 times). The product of the fusion was embedded and cultured on KM8P 1 medium. 383 calli were obtained. Of these calli, 32 shoots differentiated and 20 plantlets developed. 8 plantlets were successfully transplanted after acclimatization. Three of the plants were sterile. They set seeds after pollination with the maintainer pollen. Molecular, celluar and biochemistry analysis will be made on their progeny.