摘要
采用磷酸铅沉淀的细胞化学方法 ,电镜下观察两个抗冷力显著不同的茉莉品种幼苗ATP酶活性在细胞中超微结构定位及其经冷胁迫的变化。结果表明 ,抗寒力强的双瓣茉莉品种比抗寒力弱的单瓣品种在低温胁迫下超微结构和ATP酶活性有更强的适应能力。茉莉幼苗叶肉组织ATP酶主要定位于细胞质膜、液泡膜、叶绿体片层、线粒体脊和胞间连丝等部位 。
Ultracytochmical localization of ATP ase activity was carried out using a leading phosphate precipitation technique in the cells of two different chilling resistance Jasminum sambac L. Ait. seedlings. Under 0℃ chilling stress, the ultrastructure of cells and the ATP ase activity in the chilling resistant Jasmine (double petal cultivar) was obviously more stable than that in the chilling sensitive Jasmine(single petal cultivar). The ATP ase in Jasmine seedlings cells of mesophyll mainly located on plasmolemma, tonoplast, chloroplast lamellae, mitochondria cristae, and plasmodeslma ,whereas the ATP ase in cells of minor leaf vein mainly located on the sieve element and the companion cell.
出处
《园艺学报》
CAS
CSCD
北大核心
2001年第6期544-550,共7页
Acta Horticulturae Sinica
