石蜡包埋组织中目的基因原位逆转录-聚合酶链反应检测

Significance of detection the genome in paraffin-embeded tissue by in Situ Reverse transcription polymerase chain reaction

目的探讨原位逆转录 -聚合酶链反应技术 (ISRT -PCR)在普通制作石蜡包埋组织中的应用及价值。方法应用直接法ISRT -PCR方法对普通制作、石蜡包埋淋巴瘤组织中的丙型肝炎病毒 (HCV)基因组序列进行检测。结果从存档多年的福尔马林固定、普通制作石蜡包埋淋巴瘤组织中检测到HCVRNA序列。结论ISRT -PCR可用于福尔马林固定。

Objective To explore the application and significance of detection of purpose genome in paraffin-embeded tissue byvn Situ Reverse transcription polymerase chain reaction (ISRT-PCR). Methods The hepatitis C virus (HCV) RNA sequence was detected in general treated, paraffin-embeded lymphoma tissue by direct ISRT-PCR. Results HCV RNA sequence was detected in longtime keep with formalin-fixed, generally treated paraffin-embeded lymphoma tissue. Conclusion The ISRT-PCR can be used to detect the low copy purpose genome in formalin-fixed, generally treated paraffin-embeded tissue. This mehod provided a new way for making a full advantage of formalin-fixed, generally treated paraffin-embeded tissue.