HPV免疫组化和原位核酸杂交技术在女阴尖锐湿疣和假性湿疣诊断中的价值

The diagnostic value of HPV capsid antigen and in situ hybridization on condylomata, pseudo-condylomata in female pudendum

目的探讨HPV免疫组化和原位核酸杂交技术在女阴尖锐湿疣和假性湿疣诊断中的价值。方法通过免疫组化和原位核酸杂交技术 ,回顾性分析 2 33例女阴尖锐湿疣、假性湿疣以及湿疣样病变中HPV Ag和HPV6/ 1 1 的检出率并与病理组织学诊断作对比分析。结果 193例尖锐湿疣HPV Ag阳性 10 2例 (占 5 2 85 % ) ,HPV6/ 1 1 阳性 187例 (占 96 89% ) ,两种检测法阳性率的差异具有极显著意义 (P <0 0 1)。 2 0例湿疣样病变HPV Ag阳性 3例 (占 15 % ) ,HPV6/ 1 1 阳性 7例 (占 35 % )。 2 0例假性湿疣HPV Ag和HPV6/ 1 1 均阴性。病理上尖锐湿疣可见于诊断性挖空细胞、角化不全、棘层肥厚、基底细胞增生和上皮脚延长融合 ;而假性湿疣无诊断性挖空细胞 ,无明显棘层肥厚和基底细胞增生。结论HPV原位核酸杂交技术优于免疫组化技术 ,HPV6/ 1 1 的检测有助于鉴别尖锐湿疣和假性湿疣以及湿疣样病变。

Objective To study value of HPV immunohistochemical technique and in situ hybridization for diagnosis of condylomata, pseudo-condylomata and condylomatoid lesion. Methods HPV-Ag and HPV 6/11 were detected in vulvar condylomata, pseudo-condylomata and condylomatoid lesions of 233 patients using immunohistochemical technique and in situ hybridization. Results 193 patients were diagnosed as condylomata by histopathological examination. 102 cases were positive for HPV-Ag(52.85%), 187 positive for HPV 6/11 (96.89%). The difference of positive rate has significant( P <0.01). 20 condylomatoid lesions have 3 positive for HPV-Ag(15%), 7 positive for HPV 6/11 (35%). There are no one positive in 20 pseudo-condylomata. Conclusion In situ hybrization excelled immunohistochemical technique for HPV-Ag detecting and HPV 6/11 detecting was helpful in distinguish between condyloma acuminata and pseudo-condylomata or condylomatoid lesions.