液态发酵猴头菌多糖工艺优化研究(III)——还原糖、总糖、多糖、pH、氨基氮与培养时间的动态关系

Study on the Optimization of Polysaccharide Production from Hericium erinaceus by Submerged Culture(III) --The Dynamic Relation between Culture Time and Reducing-sugar, Total-sugar, Polysaccharide,pH Value,Amino-nitrogen

研究了液态发酵猴头菌多糖过程中还原糖、总糖、多糖、pH值、氨基氮与培养时间的动态关系。结果表明 ,还原糖随培养时间的延长呈下降趋势 ,pH值则前 2d上升然后逐渐下降 ,至 14 4h后降为 3 .8,然后维持不变 ,氨基态氮随培养时间的延长呈上升趋势 ;胞内多糖到12 0h时达最大 ,然后下降 ;胞外多糖则相反 ,培养期间持续增加。

The dynamic relation between culture time and reducing sugar, total sugar,polysaccharide,pH value,amino nitrogen are studied during submerged culture in this paper, and the results show that reducing sugar is decreasing as culture goes ahead,pH value is up during the first 2 days then down to 3.8 at 144h and suspends,amino nitrogen is increasing during culture,endocellular polysaccharide reaches maximum at 120?h then decreases, compared to endocellular polysaccharide,exocellular polysaccharide is increasing during culture.