典型黄酮类化合物在清除超氧阴离子自由基过程中产生的过氧化氢的测定方法(2)

MEASUREMENT OF THE G VALUES OF HYDROGEN PEROXIDE IN THE REACTIONS OF TYPICAL FLAVONOIDS WITH SUPEROXIDE ANION RADICALS (II)

用动态吸附的方法对几种典型的黄酮类化合物的甲酸钠水溶液 (N2 O:O2 =4:1饱和 )经γ辐照后产生的过氧化氢进行了分离 ,测定了辐照生成的过氧化氢的G值 ,得到了与前人不同的结果 ,并分析了产生分歧的原因。提出并确立了测试的方法 :准确测定黄酮类化合物辐照体系中的过氧化氢G值 ,必须先用柱色谱法将过氧化氢从辐照体系中分离出来再进行测定 ,再扣除黄酮类化合物因自氧化而产生的过氧化氢的量 ,才能得到辐照产生的过氧化氢G值。N2 O:O2 =4:1饱和芦丁、黄芩甙和儿茶素的甲酸钠水溶液经γ辐照后产生的过氧化氢G值分别为 8.3± 0 .2、5 .6± 0 .2和 7.8± 0 .2。

irradiated rutin-, catechin- and baicalin-HCOONa aqueous solutions saturated with N 2O:O 2=4:1 were eluted through alumina columns and the G values of hydrogen peroxide generated in the solutions were measured. Different results from former works were obtained and the reasons of the difference were discussed. A precise method was established as follows: hydrogen peroxide should be separated from flavonoids by passing the flavonoids solutions through alumina columns before the measurement and the amount of hydrogen peroxide generated from self-oxidation of the flavonoids should be deducted. The G values of hydrogen peroxide in γ irradiated rutin-, catechin- and baicalin- aqueous solutions saturated with N 2O:O 2=4:1 were determined to be 8.3±0.2, 5.6±0.2, and 7.8±0.2, separately.