犬全腔静脉肺动脉连接术动物模型的建立

A NEW EXPERIENTAL MODEL FOR TOTAL CAVOPULMONARY CONNECTION

为改进全腔静脉肺动脉连接术 (TCPC)动物模型的手术方法和探讨术后血流动力学变化 ,采用Y型人造血管分别与上、下腔静脉及主肺动脉行端侧吻合 ,结扎腔静脉吻合口近心端 ,术后予羟乙基淀粉代血浆维持肺动脉压在 2 0mmHg ,测量血流动力学指标。结果表明 ,所有实验犬均耐受手术 ,平均存活时间为 14h ,最长达 5天 ,术后血流动力学指标保持相对稳定 ,心排量、平均主动脉压、右 /左肺血流量比值与肺血管阻力均成显著负相关。本实验在非体外循环、心脏跳动下进行 ,且不缝闭三尖瓣和肺动脉瓣 ,避免了心内操作 ,方法简便 ,术后存活时间长 。

To establish a new model of total cavopulmonary connection (TCPC) by improving the vascular anastomosis between vena cava and pulmonary artery and to study the postoperative hemodynamic changes, a Y shaped prosthetic blood vessel was used, one limb of the prosthesic was anastomosed with vena cava, and the other to pulmonary artery in an end to side way. Then the proximal vena cava was ligated. The mean pulmonary artery pressure was kept at about 20mmHg, the hemodynamic parameters were measured. All dogs tolerated the operative procedure. It was found that pulmonary vascular resistance (PVR) showed a significantly negative correlation with cardiac output (CO), mean aortic pressure (mAoP), and the ratio of right/left pulmonary artery flow (RPF). The model was successfully developed without cardiopulmonary bypass, and the tricuspid valve and pulmonary artery valve were left untouched. Hemodynamic parameters remained stable. It will be useful in study of the circulation after TCPC operation.