斑点免疫吸附试验诊断羊脑多头蚴病的研究

Study on the Application of Dot-ELISA for Diagnosing Coenurosis in sheep

以原头节可溶性粗抗原经 Ｓｅｐｈａｄｅｘ Ｇ－２００层析纯化抗原为包被抗原，兔抗羊 ＩｇＧ－ＨＲＰ结合物为显色剂，建立检测羊脑多头蚴病血清抗体的 Ｄｏｔ－ＥＬＩＳＡ，并以 ＥＬＩＳＡ作平行对照。结果，粗抗原和层析纯化抗原检测 ８６头份羊脑多头蚴病阳性血清，其敏感性分别为 ９４．１８％和９３．０２％，两种抗原的敏感性无显著差异；检测１２２头份绦虫蚴病阴性血清，１８头份棘球蚴病阳性血清，３５头份细颈囊尾蚴病阳性血清，其特异性分别为９０．２９％和９５．４３％。两种抗原的特异性差异显著。Ｄｏｔ－ＥＬＩＳＡ和ＥＬＩＳＡ两种方法的符合率为１００％。层析纯化抗原比粗抗原的特异性有了明显提高，而敏感性没有降低。层析纯化抗原和操作术式都具有良好的重复性，Ｄｏｔ－ＥＬＩＳＡ和 ＥＬＩＳＡ对比试验结果相近，且具简便、快速及不需要复杂设备等优点，是一种检测羊脑多头蚴病血清抗体的理想方法。

The antigen that filter-puritied by Sephadex G-200 with protoscolex ES unpuritied antigen is covered antigen. The mixture of Rabbit anti-goat IgG-HRP is regent, Establish a Dot-ELISA for detecting sera antibody of Coenurosis and use it as a parallel contrast. The result is that we use unpuritied antigen and filter puritied antigen to detect 86 positive serum of Coenur, its sensitivity is 94.18% and 93.02% respectively. There is significant difference between two antigens. Detect 122 negative sera of Proscolx, 18 positive sera of Echinococcus, 35 positive sera of Cysticercus tenicollis. Their specificity is 90.29%, 95.43% respectively. The different of specificity between two antigens is significant . Fitting rate of two methods of Dot-ELISA and ELISA is 100%. Filter puritied is more higher. But sensitivity isn't lower. Both filter purified antigen and operating methods can repeat well. The contrast results of Dot-ELJSA and ELISA are similer and has the advantages of fast, simplicity and without specific equipment. It is an ideal way for detecting sera antigen of Coenurosis of sheep.