雌激素治疗局限性脑缺血和性别因素的影响

Effects of gender aud estradlol treatment on focal brain ischemia

摘 要：目的 探讨雌激素对雄性大鼠和雌性大鼠局限性脑缺血的治疗作用和性别因素的影响。方法 应用局限性脑缺血动物模型。结果 实验1组实验大鼠阻断大脑中动脉（MCA）后的死亡率：正常雄鼠和雌鼠均为12．5％（2/16），去卵巢雌鼠为23．5％。冠状面的脑梗死范围（平均值±标准差）：正常雌鼠为9．5％±1．0％，正常雄鼠为16．6％±1．6％（两者相比P＜0．01），去卵巢雌鼠为16．0％±1．4％（与正常雌且相比P＜0．01）。实验2组实验大鼠在阻断MCA 40min后给去卵巢雌鼠注射雌二醇（E2）或其溶液羟丙基-β-环右旋聚糖（HPCD）。HPCD治疗组死亡率为40％（4/10），雌激素治疗组为0％。脑梗死的范围：HPCD治疗组为19．3％±1、8％，雌激素治疗组为8．0％±1．2％（两者相比 P<0．01）。血清中雌激素含量：HPCD治疗组为 14．5±1．2pg/ml，雌激素治疗组为142．7±23．6pg／ml（两者相比 P<0．01）。结论 在 MCA阻断造成局限性脑缺血时，雌激素有保护脑缺血的作用。

Abstract: Objective This paper was undertaken to investigate the effects of gender and estrogen treatment on focal cerebral ischemia in male and female rats. Methods Focal brain ischemia animal model was used. Results In first study, the mortalities from middle cerebral artery(MCA) occlusion were 12. 5% each for intact male and intact female rats, and 23. 5% for ovariectomized (OVX) female rats. The coronal infarct area was 9. 5%?. 0% for intact female rats,16. 6%?. 6% for intact male rats (P<0. 01 vs. intact female rats),and 16. 0%?. 4% for OVX female rats (P<0. 01vs. intact female rats). In second experiment, OVX-female rats were administrated either 17B-estradiol (E2) or its vehicle, hydroxypropyl-B-cyclodextrin (HPCD), at 40 min after the onset of MCA occlusion. Mortalities were 40% for vehicle treated OVX rats and 0% for E2 treated OVX rats. The infarct area was 19. 3%?. 8% for vehicle treated rats vs. 8. 0%?. 2% for E2 treated OVX rats (P<0. 01). Conclusion These results strongly suggest that the level of circulating estrogens play an important role in protecting brain tissues against ischemia induced by MCA occlusion.